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KMID : 0613820140240090946
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Journal of Life Science
2014 Volume.24 No. 9 p.946 ~ p.951
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Effect of Ulmus macrocapa Ethanolic Extracts on Anti-oxidant Activity and Melanin Synthesis in B16F1 Cells
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Kwon Eun-Jeong
Park Hye-Jung
Kim Moon-Moo
Lee Kyung-Rok
Hong Il
Lee Do-Gyeong
Oh Yung-Hee
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Abstract
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Melanin plays a key role in the protection of skin from ultraviolet light that generates reactive oxygen species (ROS), such as superoxide, hydroxyl radical, singlet oxygen and hydrogen peroxide. However, the ROS leading to the oxidation of lipids, proteins and DNA are involved in the overproduction of melanin that is known to cause melasma, age spots and freckles. Among the herb medicines, Ulmus macrocarpa used in this study was reported to contain flavonoids as a main component. The aim of this study is to investigate the whitening and anti-oxidant effects of Ulmus macrocarpa ethanolic extracts (UMEE) in B16F1 cells. UMEE below 3.12 μg/ml did not show cytotoxicity. In an anti-oxidant experiment, UMEE showed not only high reducing power and scavenging activity on DPPH, but it was also observed that UMEE exhibit an inhibitory effect on lipid peroxidation. UMEE did not display an inhibitory effect on tyrosinase activity in vitro. However, UMEE inhibited melanin synthesis in B16F1 cells. In addition, UMEE reduced the expression levels of tyrosinase and tyrosinase-related protein-2 (TRP-2), which are key enzymes in melanogenesis. These results indicate that UMEE exert a whitening effect through the inhibition of both tyrosinase and TRP-2 expressions as well as anti-oxidant activity, suggesting that UMEE could have the functional potential for a whitening effect on the skin.
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KEYWORD
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B16F1, TRP-2, tyrosinase, Ulmus macrocarpa ethanolic extracts (UMEE), whitening
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